COURSE DESCRIPTION

This interactive three-day course includes hands-on use of advanced microscopes, slide preparation, and filament staining techniques. Students will learn proper care and use of microscopes, as well as activated sludge process testing and troubleshooting. The course focuses on the valuable information a microscope provides for controlling and troubleshooting an activated sludge wastewater treatment plant. Students are encouraged to bring small MLSS samples from their facility to use during slide preparation and organism identification exercises.  Students prepare gram and neisser stains for filament identifcation and learn wet mount slide preparation techniques. Students will receive the book "A Wastewater Microbiology Laboratory Manual for Operators" a $132 value and also includes a DVD of live video clips along with color images of many of the microorganisms and procedures mentioned in this book to assist you as you develop your microbiological skills. 

Topics covered include:

• Using the microscope as a process control tool
• Identifying bacteria, protozoa and filaments
• Learning staining techniques
• Interpreting results

WHO SHOULD ATTEND

This is an excellent course for intermediate to advanced level operators.

ADDITIONAL INFORMATION

Planning to bring a sample to the Microbiology class?

Here’s what to do:

• Use a small (100 mL) plastic bottle with a secure lid
• Collect sample from normal location for grabbing MLSS settleability test
• Collect sample as close to the day of travel to TREEO as possible (fresher is better)
• Add about 20 mL of fresh MLSS to bottle, secure lid
• DO NOT add preservatives
• Be sure there is adequate air space (aerobic organisms like oxygen)
• Place sample in a Ziploc bag for extra leak proofing
• Write date and time and location on bottle or bag, or both
• No need to place on ice if viewing within 12 hrs.
• You are welcome to bring multiple samples from various locations, just follow above guide
• Still confused? See photo below!

STUDENT TESTIMONIALS

"I don’t know what else to give this course other than a excellent +. I must admit I mostly was interested in getting my CEU’s, however; this is the only 2-day course I have taken for CEU’s that I wish I could stay longer.”

“Recommended this class to all WW operators.” 

INSTRUCTOR'S BIOGRAPHY

Ron Trygar, CET

Ron is the Senior Training Specialist for Water and Wastewater programs at the University of Florida TREEO Center.  Previously he was the Wastewater Process Control Specialist at Hillsborough County Public Utilities in Tampa FL.  He has worked in the wastewater industry for more than thirty years in a variety of locations and positions. Ron became a Certified Environmental Trainer (CET) in 1998 and has since provided training for associations and regulatory agencies such as FDEP; FW&PCOA Short Schools; USABlueBook; FWEA sponsored training events and local school environmental programs. Working alongside the FDEP Northeast District, Ron helped begin the FRWA/FDEP joint operator certification review classes that are still given around the state today.  Ron holds a Florida class A wastewater treatment operator’s license and a Florida class B drinking water operator’s license.

Link to Instructor Biographies

*One student per registration fee, CEUS will only be reported for registered students

*Please submit your license type/number before the last day of course instruction